Coding

Part:BBa_K4813001:Design

Designed by: Tracy Chu   Group: iGEM23_HongKong-JSS   (2023-10-03)


tdTomato - red fluorescent protein codon optimized for E. coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1066
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1066
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1066
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1066
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3


Design Notes

Nil


Source

The original tdTomato sequence was obtained from NCBI. Then, the sequence was codon optimized by IDT and gBlock was synthesized from IDT.

References